[ccp4bb] size of protein in negatively stained TEM?

Apologies for the non-crystallographic question, but I would think the wide experience and vitality of this board might have some opinions on this topic. We've sent out some samples for negative staining electron microscopy. They were stained with uranyl acetate on commercial grids and the result

Re: [ccp4bb] Catalytic residues in active sites

On 15:52 Tue 25 May , Clayton, Gina Martyn wrote: > I wonder if anyone can recommend a good review/paper describing > crystal structures that show high energy residues in active sites. By > that I mean residues that may be in a strained conformation and rotate > between conformations, such t

Re: [ccp4bb] delta delta G color coding

Dear Ivan, If I understand correctly what you want to achieve, one common way is to abuse the B-factor column and replace those values with the values you want to present. Tim On Thu, May 27, 2010 at 12:07:23PM -0700, xaravich ivan wrote: > Hi, > Apologies for a non CCP4 questions but this one i

[ccp4bb] delta delta G color coding

Hi, Apologies for a non CCP4 questions but this one is definately for crystallographers, I have a set of delta delta Gs for there different protein ligand interactions.I want to show them in pyMOL. Is there any program or script that I could run such that the output will be read by Pymol and color

Re: [ccp4bb] Protein-DNA complex crystallisation

Hi Jhon, Here are a few hints for protein-DNA crystallization: 1.) Starting point for protein:DNA ratio is 1:1.2 for a tight binder. In your case it would be protein complex to DNA 1:1.2. I like to run size-exclusion chromatography (if applicable) to determine an optimal ratio with a slight excess

[ccp4bb] Postdoctoral position at the Curie Institute PARIS - for a protein crystallographer

A post-doctoral position is available in the “Structural Motility” Team directed by Dr. Anne Houdusse at the Curie Institute, Paris, France. Our research project includes structural studies of molecular motors in close collaboration with functional studies. Our goal is to understand how the

[ccp4bb] Scientific Programmer Position

Scientific Programmer Position The Nucleic Acid Data Project at Rutgers The State University of New Jersey is seeking candidates for a scientific programmer position to support development of new tools and database services for RNA structure analysis. The position requires: Minimum of Bachelor

[ccp4bb] Murnau Conference 2010 on Structural Biology of the Modern RNA World (13-16 October, 2010)

Dear colleagues, the Murnau Conference 2010 on Structural Biology of the Modern RNA World will be taking place from 13 to 16 October, 2010. As you might already know, the Murnau Conference is an international meeting that covers medically relevant and timely topics in the wide field of modern

Re: [ccp4bb] Fwd: calculate the real space R factor using OVERLAPMAP

On Tue, May 25, 2010 at 6:58 AM, Pavel Afonine wrote: > Yes, because all the information you need is encapsulated in 1 number > per region of interest! > > I agree it's a good reason. Actually what I described doesn't quite achieve this, because you need to keep a record of both the RMSD Z-score

Re: [ccp4bb] Lindemann glass

Hilgenberg (Malsfeld, Germany) currently offer their remaining stock. Clemens Zitat von James Holton : Does anyone know the formula or supplier of what was once called "Lindemann glass"? It is a silicon-free high-boron glass that has no elements in it heavier than oxygen. Used to be used

[ccp4bb] Lindemann glass

Does anyone know the formula or supplier of what was once called "Lindemann glass"? It is a silicon-free high-boron glass that has no elements in it heavier than oxygen. Used to be used to make clear x-ray windows, but now I can't find anyone who sells it. -James Holton MAD Scientist