Hi Kristof We had an example a few years back, with a Co ion leakage off an old Talon resin necessary for the crystal to grow (until we realized you could just add Co instead to get crystals to grow).
The ion was used in the end as anomalous scatterer to solve the structure, and was found bound nicely between two molecules in the crystal. Guncar et al, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):209-13 http://www.ncbi.nlm.nih.gov/pubmed/17329816 Bostjan On 10/12/10 9:17 PM, "Kristof Van Hecke" <kristof.vanhe...@chem.kuleuven.be> wrote: > Dear, > > I was wondering if anybody has experienced before the leakage of Ni-ions (from > a Ni-NTA column) and additionally binding to specific sites in the protein > structure..? > > Many thanks > > Regards > > Kristof > > > -------------------------------------- > Kristof Van Hecke, PhD > Biomoleculaire Architectuur > Celestijnenlaan 200 F > B-3001 Heverlee (Leuven) > Tel: +32(0)16327477 > -------------------------------------- > > > > > --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences > and Institute for Molecular Bioscience (Division of Chemistry and Structural > Biology) and Centre for Infectious Disease Research Cooper Road University of Queensland Brisbane, Queensland 4072 Australia Phone: +61 7 3365 2132 Fax: +61 7 3365 4699 E-mail: b.k...@uq.edu.au URL: http://www.scmb.uq.edu.au/staff/bostjan-kobe Office: Building 76 Room 329 Notice: If you receive this e-mail by mistake, please notify me, and do not make any use of its contents. I do not waive any privilege, confidentiality or copyright associated with it. Unless stated otherwise, this e-mail represents only the views of the Sender and not the views of The University of Queensland.
