Dear Israel,
as Martin pointed out we have a device here at the ESRF/EMBL, the
HC1b, that produces a stream of air with a precisely controlled RH at
the sample position that we have used with some success to monitor the
effects dehydration has on diffraction quality. The same device is
On Mon, 2011-05-02 at 15:48 +0800, anita p wrote:
What are the reasons behind nonreproducibility of protein crystals?
There are many possible reasons. You could start by checking you
protein stock for signs of degradation and preparing it afresh.
--
Hurry up before we all come back to our
Anita,
Proteolysis and oxydation are the most common alteration affecting proteins
during the course of crystallization.
If you have drops of the trays that yielded crystals I would run a gel on
those drops and look at the aspect of protein still around in the drop. That
would give you some
Dear George,
I really appreciate your kind offer but I have just solved
the problem.
To all that may have the same issue in the future, I
should say that in my case the problem was related to the
use of SHELXPRO. I was not using it properly to transform
the chains read by SHELXL (numbered)
Dear Fatima,
Thanks. I am anyway planning to allow chain identifiers in the next
version of SHELXL.
Best wishes, George
Prof. George M. Sheldrick FRS
Dept. Structural Chemistry,
University of Goettingen,
Tammannstr. 4,
D37077 Goettingen, Germany
Tel. +49-551-39-3021 or -3068
Fax.
Hi Matt,
I'm trying to 'measure' the density in a region of my structure by
integrating the electron density there (notwithstanding errors in F000). I
understand that both overlapmap and phenix.real_space_correlation compute
density surrounding individual atoms for their calculations. Is
The CCP4 program that makes the label map you are looking for is SFALL. It
can be told to make a .map file where each grid point is still a floating-point
number, but instead of the usual electron density it encodes the residue
number, atom number, etc. The OVERLAPMAP program knows how to
On Mon, May 2, 2011 at 12:53 PM, Pavel Afonine pafon...@gmail.com wrote:
Hi Matt,
I'm trying to 'measure' the density in a region of my structure by
integrating the electron density there (notwithstanding errors in F000). I
understand that both overlapmap and phenix.real_space_correlation
On Mon, May 2, 2011 at 2:18 PM, James M Holton jmhol...@lbl.gov wrote:
The CCP4 program that makes the label map you are looking for is SFALL.
It can be told to make a .map file where each grid point is still a
floating-point number, but instead of the usual electron density it
encodes the
Hi Matt,
What I want is the density 'inside' each atom in the map, so that I can make
quantitative comparisons of how much stuff is present in a blob (modeled
or not).
that's still ambiguous...: 1) do you want a density value calculated at a
point, which may be, for example, a center for an
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