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Dear Jacob,
Coot prints this information to the terminal, so you can start coot and
'tee' its output into a file.
Tim
On 11/28/2011 11:53 PM, Jacob Keller wrote:
Let me refine my question (sorry for my lack of clarity): is there a
program that
Dear Christine,
I had guessed that you had more salt in the protein solution
than in the reservoir with a relatively low protein concentration.
The protein is in:
100mM Sodium Acetate pH 5.5 with 150mM NaCl at a protein concentration
of ~4.3mg/mL.
and the reservoir is:
(0.05M Calcium
Hi CCP4 community.
While running the Phaser in the CCP4 GUI, It was terminated and giving the
error message.
#CCP4I TERMINATION STATUS 0 child killed: segmentation violation
#CCP4I VERSION CCP4Interface 2.0.6
CCP4 Version ccp4-6.1.13
Thanks in advance.
Sita Ram
The Biozentrum of the University of Basel, an internationally renowned
Life Sciences research institute, invites applications for the Leader of
its new Biophysics Facility. We are looking for an enthusiastic person
that combines technical expertise with communication skills. The ideal
candidate is
Postdoctoral position,
Institut
de Biologie Stucturale, Grenoble, France
A
two year postdoctoral position for a biochemist/structural
biologist
is available in the Synchrotron Group at the Institute for
A 2 year postdoctoral position with support from the French National
Research Agency (ANR) is available to study regulatory mechanisms of
BMP-1/tolloid like proteinases ((BTPs; also known as procollagen
C-proteinases) which are enzymes implicated in a number of key events
during tissue
Christine, I have a couple more comments
First, if those crystals come back, I would certainly try microseeding into
*random *screens. You should be able to pick up new conditions that have a
little more salt in them, and are therefore stable regarding the movement
of water. (If you can't get
Dear All,
Apologies for the off-topic question. I'm seeking suggestions on the best
way to achieve an effective, but not over-harsh, chemical lysis of E coli
expressing an oxygen-sensitive Fe-S protein. We need to lyse anaerobically
but do not have access to a sonicator that we can use in
0.5% octyl-thioglucopyranoside + lysozyme + DNAse in 30 mM TRIS pH 8.0 -
degassed/sparged and supplemented with whatever reducing agent you need.
Stir in cold for 30-40 minutes at a ratio of 3ml of solution to 1g of cell
pellet (wet).
Artem
On Tue, Nov 29, 2011 at 8:49 AM, Jim Spencer, Cellular
Dear Crystallographers,
Thanks very much for all who responded to my request. Below is a
compiled list of the various ways to skin this crystallographic cat!
Jacob
-Moleman ca plot distance-CCP4's superpose-distance matrix analysis,
e.g.
Dear Crystallographers,
does anyone have any nice examples/references of proteins which form
demonstrably physiologically-relevant oligomers in crystals, but which
do not appear to do so in solution? I am thinking particularly that
domains of membrane proteins which oligomerize primarily through
To whom it may concern,
I am writing this in request to the cancellation of my
subscription to CCP4BB mail.
It was indeed a very rewarding and a knowledgeable experience
to be a part of this family.
With regards
Debanjan Choudhuri
With such a nice parting letter, we find it disheartening to let you go.
James
On Nov 29, 2011, at 7:44 PM, debanjan choudhuri wrote:
To whom it may concern,
I am writing this in request to the cancellation of my subscription to CCP4BB
mail.
It was indeed a very rewarding and a
Just you wait--he'll be back...won't he?
On Tue, Nov 29, 2011 at 11:11 PM, James Stroud xtald...@gmail.com wrote:
With such a nice parting letter, we find it disheartening to let you go.
James
On Nov 29, 2011, at 7:44 PM, debanjan choudhuri wrote:
To whom it may concern,
I am writing
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