Hi Lucas,
The funky diffraction pattern is most likely due to a cracked crystal,
resulting in a mixture of slightly differently aligned diffraction
patterns. Were the cracks there before you added the cryprotectant? If
not, the cryoprotectant is definitively to blame. As has mentioned
before, you
An earlier post said the point-group is P2, and these reported cells do not
quote the beta angles: what are these angles?. In the monoclinic system it is
possible to have two closely-similar alternative cells in certain special
cases, and if the crystals have been indexed differently this could
Dear ccp4
I ask a very fundamental question because I have not had formal training in
this and I would like to understand.
How can I obtain the multiplicity (z) from the space group? So for example if
the space group is P222 how do I know that there are 4 monomers in the unit
cell? Or if it
Hi Careina
The obvious answer is to look it up in International Tables vol. A.
If you don't have access to that you can look up the text file
$CLIBD/syminfo.lib in the CCP4 distribution and work it out from
there. Fpr a given space group you need to count the number of
'symop' lines. That's the
I don't want to confuse things further, but as a PS to Ian's answer that
clearly tells you how to get Z..
You should be aware that a crystal might also have non-crystallographic
symmetry.
Ian's answer is right for Z, but as you also mentioned monomers I thought
I should mention that if the
Dear colleagues,
this is to remind you that the Murnau Conference 2012 on Structural Biology of
Molecular Transport will be taking place from 17-20 October. Online
registration is OPEN (http://www.murnauconference.de/2012/registration.html).
We would be delighted to meet you at the conference.
Dear All:
Thank you very for your comments and advices. '
I am getting to know why are the problems. And will try again.
I appreciate you all for your inputs
regards
Uma
On Thu, Aug 2, 2012 at 4:11 AM, Phil Evans p...@mrc-lmb.cam.ac.uk wrote:
An earlier post said the point-group is P2, and
Uma,
Before this discussion goes much further, you need to provide more details:
1) putative space group?
2) observed resolution and diffraction anisotropy?
3) how big was the crystal and what was its shape? Was the crystal split?
4) were the data sets taken at different points on the
Dear Colleagues,
I have a question for all of you bioinformatics oriented structural biologists:
How do I predict the sites of protein-protein interactions between two
receptors that have been proven to interact biochemically but lack specific
details regarding proximity. This is not a
Hello ccp4BBers,
Our lab is thinking about trying to purchase a crystallization robot.
We need to be able to do LCP in addition to sitting drop experiments. Does
anyone have experience with the NT8 robot from formulatrix? The other two
robots I am aware of are the LCP Mosquito from TTP and
Dear Sam,
I spent quite some time trying to get up and running a HT LCP setup in the
past years, and all I can say is that TTP has successfully automated in its
Mosquito LCP all the homemade workarounds I employed back then. For
instance, they tackle the dehydration problem by covering the drop
*CALL FOR PROPOSALS FOR ESRF BEAM TIME WITH ONLINE MICROSPEC*Proposal
Deadline *4th September 2012*
There will be beam time available at the ESRF for MX data collection
with a setup that allows online monitoring of UV/VIS absorbance or
fluorescence spectral changes of the crystal during the
On 08/02/2012 04:37 AM, Careina Edgooms wrote:
Dear ccp4
I ask a very fundamental question because I have not had formal
training in this and I would like to understand.
How can I obtain the multiplicity (z) from the space group? So for
example if the space group is P222 how do I know that
The space group decoder does exactly these steps and lists additional useful
information.
http://www.ruppweb.org/new_comp/spacegroup_decoder.htm
Other examples including unit cell packing in C2 are in BMC chapter 5.
Best, BR
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Hi,
cctbx Explore symmetry will do this and lot more:
http://cci.lbl.gov/cctbx/explore_symmetry.html
Pavel
On Thu, Aug 2, 2012 at 1:37 AM, Careina Edgooms careinaedgo...@yahoo.comwrote:
Dear ccp4
I ask a very fundamental question because I have not had formal training
in this and I would
Also I see it works on all settings, not just the limited set of
standard symbols, doesn't need spaces in the names (which are
redundant anyway), and uses the correct xHM symbols (such as R32:r).
It also accepts the PDB-only symbols H3 H32.
Well done Pavel - Like + :).
Cheers
-- Ian
On 2
Hi Lorenzo,
If the structure for your receptor is unknown, then you can use Homology
Modeling methods to get a rough idea of the structure, MODELLER is a well know
tool for this (http://salilab.org/modeller/). Of course depending on your %
similarity to the template, the higher the %
Hi Herman and other CCP3BBers,
Thanks for your suggestions. I didn't see any cracks in the crystal drops
initially. I will certainly try to shot crystals under room temperature and see
what happens. Does the plastic loops fit into the cryo stands Molecular
Dimension sells?
LUcas
On Aug 2,
Mitegen makes a nice little product that is a plastic tube that will slide
over one of their magnetic cap/loops. If you put some well solution in the
tube and seal the base with apiezon, you can collect quite a bit of data on
the loop mounted crystal before it dries out.
Cheers,
We have a Crystal Gryphon and it works fine for sitting drop plates. We
usually set 200+200 nL drops for screens, but it can of course dispense
much larger volumes if required. It's affordable and easy to maintain, not
much in the way of consumables required. The software is very easy to use,
and
I do not have the reference you are seeking, but I have seen
cacodylate-containing xtals diffract to better than 1.2 and hold up very
well. Also, arsenic has an anomalous signal which may be exploited for
phasing, peak ~ 1.04 A.
On 07/29/12 18:53, Tatyana Sysoeva wrote:
Hi!
I heard a
A few thoughts:
1. Search for all possible space groups (e.g., P2 and P21 in this
case). Be happy it isn't C222, which means 8 possible combinations
of screw axes to search! As mentioned already, P21 is far more
common than P2. I think P21 is one of the most common space groups
in
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