Dear Yuan Shang,
HSSP provides multiple sequence alignments with conservation scores per
position. It is originally PDB derived in the sense that a multiple sequence
alignment already exists for each PDB entry. You can also make HSSP entries
from sequence alone, but you should contact the HSSP
Dear Theresa,
You could try to add 50 mM of L-Arg and 50mM L-Glu to your buffers during
purification. These amino acids are effective in preventing protein aggregation
and precipitation and protect samples from proteolytic degradation.
Cheers,
Arnaud
On Aug 21, 2012, at 10:15 PM, Theresa
