Re: [ccp4bb] ionic interaction inside a protein

2013-03-25 Thread Seema Nath
The link is fine. If you still get the 'error message', then google PIC webserver , fullform of PIC - protein interactions calculator. Seema Nath

[ccp4bb] cell disruptor / homogenizer

2013-03-25 Thread Clemens Steegborn
Dear colleagues, We are currently looking for new cell disruptor/homogenizer equipment, mainly for E.coli work. We currently have a Branson sonifier and a Microfluidics Fluidizer - the latter one keeps causing trouble, and we think about a replacement. We previously also used an

Re: [ccp4bb] cell disruptor / homogenizer

2013-03-25 Thread Barbara Giabbai
Dear Clemens, I used the Panda homegenizer (GEA Niro Soavi) to disrupt E.coli cells: is really good, I always did the maintenance on my own, is not very expensive and easy to use. I found bigger models also in the big pharmas...so is really reliable. http://www.niro-soavi.com/home.html

[ccp4bb] Software developer position

2013-03-25 Thread Jose A. Marquez
Dear All A Software Developer position is currently available in my laboratory at EMBL Grenoble. You will find the details in the link below or at www.embl.org/jobs http://www.embl.fr/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670

[ccp4bb] Call for targets of biomolecular complexes for CAPRI

2013-03-25 Thread Chris Morris
Members of this mailing list may be in a position to help with targets for the next Critical Assessment of PRediction of Interactions . CAPRI (Critical Assessment of Predicted Interactions) is a community-wide blind experiment aimed at assessing the performance of protein docking algorithms

[ccp4bb] השב: Re: [ccp4bb] Vote for crystallography

2013-03-25 Thread Boaz Shaanan
Well done to you Fred for pointing this out to us. How did you comr across it? Cheers, Boaz ‏נשלח מהטלפון הנייד של Samsung הודעה מקורית מאת: vellieux frederic.velli...@ibs.fr תאריך: אל: CCP4BB@JISCMAIL.AC.UK נושא: Re: [ccp4bb] Vote for crystallography Dear

Re: [ccp4bb] molecular replacement problem.

2013-03-25 Thread Eleanor Dodson
Umm - this is tricky. First of all you need to reindex the C2221 data into the P21 cell - do you know the operator? then expand that data set to spacegroup P21. There is a cad option to do this.. Then add that FreeR to the re-processed P21 data. Eleanor On 24 March 2013 14:37, Appu kumar

Re: [ccp4bb] ionic interaction inside a protein

2013-03-25 Thread Faisal Tarique
Thanx everybody..PICserver is really good On Mon, Mar 25, 2013 at 12:09 PM, Seema Nath seema.n...@saha.ac.in wrote: The link is fine. If you still get the 'error message', then google PIC webserver , fullform of PIC - protein interactions calculator. Seema Nath -- Regards Faisal

Re: [ccp4bb] Strange density in solvent channel and high Rfree

2013-03-25 Thread Eleanor Dodson
First - I dont think you have a 3rd molecule where you have put it - or at least not one with full occupancy. Those maps are a clear indication that something is wrong. What is the Matthews coefficient for the numbers in the asymmetric unit? Presumably your processing gave you a lattice which