Hi, I'm meant to know this but I'm blanking, so I'll crowdsource instead:
Anybody know a (the) reference where it was showed that the best SAD
data is obtained by collecting multiple revolutions at different crystal
offsets (kappa settings)? It's axiomatic now (I hope!), but I remember
seeing
-- Forwarded message --
From: megha goyal
Date: Mon, May 6, 2013 at 1:16 PM
Subject: bioassay g-csf
To: CCP4BB@jiscmail.ac.uk
we perform bioassay of g-csf using m-nfs-60 cell lines but we do not get
reproducible results. some time a sample fails and on repeating the assay
for sam
On 05/13/2013 06:34 PM, Evgeny Osipov wrote:
Hello everybody,
I am trying to evaluate solvatation energy of my protein and.
Unfortunately the protein highly glycosilated and it seems that PISA
does not take into account hydrogen bonds between mannose from one
molecule and symmetry related molecul
Hi Joel
IF you use external restraints then you need to add type 0 at the end. Then
refmac will assume that you want to override standard restraints. I.e.
exte angle first chain A resi 1 atom S12 second chain A resi 1 atom C10 third
chain A resi 2 atom N value 120 sigma 3.0 type 0
It is very st
H ithere,
I am trying to refine a covalently bound ligand to my protein and I am having
trouble with the restraints. I have generated the cif file and link within
Jligand and this is reasonable. However it appears that REFMAC is overriding
these and fitting the ligand to the density.
I have ad
Sometimes a floppy bit of a protein is even more floppy in a
particular crystal form. Your maps do not appear to support your
model of a helix in this location. I would not build it unless
maps based on later refinement show something reasonable in the
omit map. (Of course if you leave out t
The Page Laboratory at Brown University has an opening for a highly
motivated postdoctoral research scientist to undertake structural analysis
of signaling complexes, with a focus on the interactions between
phosphatases, kinases and their regulatory proteins. Most studies typically
employ a combin
Dear all,
Opening for Associate/Assistant Professor Position in the Department of
Biophysics, National Institute of Mental Health and Neuro Sciences
(NIMHANS), Bangalore, INDIA.
Research experience: preference to Neuroscience/membrane proteins.
Here is the link to the advertisement:
http:
Hello everybody,
I am trying to evaluate solvatation energy of my protein and.
Unfortunately the protein highly glycosilated and it seems that PISA
does not take into account hydrogen bonds between mannose from one
molecule and symmetry related molecule.
Is there any way to tell PISA to use lig
Hard to say without seeing the maps and experimenting. My first check would
be to set the NTD occupancies to 0.0 - refine the CTD alone, then look at
the maps in COOT.
Or maybe let an automatic modelling building program such as Buccaneer try
to rebuild the NTD section, with starting phases from t
MRC Laboratory of Molecular Biology, Cambridge, UK
Post-doctoral Scientist Position
We seek to appoint a postdoctoral researcher to the Division of Structural
Studies to undertake protein crystallographic analysis of the anaphase
promoting complex (APC/C) and its subunits. This is an Invest
Dear all,
I have solved the structure of my protein by molecular replacement at 2.9A,
with Rfactor and Rfree 18 and 22 respectively. Overall everything seems
fine, its a two domain protein NTD and CTD, the NTD have high average B
factor compared to CTD. A helix of NTD seems to be disordered, I tri
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