[ccp4bb] CCP4 Support 32 bit OSX - request for feedback

2013-07-13 Thread Eugene Krissinel
PPS So far we have received a VERY limited response to this call. Please see a nice hint from Bill Scott if you are in doubt whether your Mac is capable of running 64-bit applications: Hi Eugene: If there are many people who think they need 32-bit support, you might want to request they issue

Re: [ccp4bb] ctruncate bug?

2013-07-13 Thread Ian Tickle
On 8 July 2013 18:29, Douglas Theobald dtheob...@brandeis.edu wrote: That's all very interesting --- do you have a good ref for TDS where I can read up on the theory/practice? My protein xtallography books say even less than SJ about TDS. Anyway, this appears to be a problem beyond the

[ccp4bb] Concentrating purified membrane protein

2013-07-13 Thread Raji Edayathumangalam
Hi Folks, Sorry for the non-ccp4 post. I have purified an 18kDa membrane protein and want to concentrate the protein from gel filtration fractions, which are in buffer containing 0.05% DDM (well above the CMC for DDM). My colleague was able to concentrate a 30kDa membrane protein using a 100kDa