[ccp4bb] BSR-2013 News and A call for BSR-2016 bids

2013-07-16 Thread margret
Dear All, with this mail we would like to draw your attention to the upcoming 11th Biology and Synchrotron Radiation Conference in Hamburg ( Sunday 8th September until Wednesday 11th September 2013). We were able to compile an exciting Conference Programme which is now available at

[ccp4bb] [ot] looking for Crystal Farm 400 service maintenance

2013-07-16 Thread Sebastiano Pasqualato
Dear all, sorry to bother you with an off-topic request. Does anybody have a contact for a person or a firm that provides maintenance and service for Crystal Farm plate imagers? They used to be serviced by Bruker, but they don't support them anymore, as far as I know. Any help highly

Re: [ccp4bb] frosted crystals during storage in pucks

2013-07-16 Thread Edward Lowe
At Elspeth's request, here's a link to a picture of the rack we use for drying out shipping dewars. https://www.dropbox.com/s/8ber2iikra9x73y/2013-02-21%2013.39.38.JPG Very simple and both we and the Lea group here have found it to make a huge difference the the effectiveness and longevity of

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread Bosch, Juergen
Special position ? sulfate-size ? Jürgen On Jul 16, 2013, at 11:35 AM, Wei Feng wrote: Dear all, I found some redundant density in my structure beween two molecule. (see picture 1 and 2) But I am not sure which ligand will be. Dose everyone see this ligand before? If so, can you tell the PDB

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread Oganesyan, Vaheh
It actually looks much like pyrophosphate! If your protein is phosphatase and the extra density is in vicinity of the active site it might be the remaining product of reaction. Vaheh From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread Nicolas Foos
Dear wei, i think you have to keep in mind what do you have in your cristal growing condition, and in you protein buffer. Because maybe you can find which molecules this ligand could be. It's difficult to help more, because we don't know you level of sigma for the map and the resolution of

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread Bosch, Juergen
Yes that was what I was thinking as well, that's why the size-comparisson. Jürgen On Jul 16, 2013, at 11:56 AM, Oganesyan, Vaheh wrote: It actually looks much like pyrophosphate! If your protein is phosphatase and the extra density is in vicinity of the active site it might be the remaining

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread Vandna Kukshal
Its look like pyrophosphate to me . wts you protein's nature is this phosphatase or ATPase ?? vandna On Tue, Jul 16, 2013 at 11:19 AM, Bosch, Juergen jubo...@jhsph.edu wrote: Yes that was what I was thinking as well, that's why the size-comparisson. Jürgen On Jul 16, 2013, at 11:56 AM,

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread sonali dhindwal
hi Wei, This looks like MPD, 2-Methyl-2,4-pentanediol. May b u have added it as ur cryo-protectant. Thanks and Regards   -- Sonali Dhindwal “Live as if you were to die tomorrow. Learn as if you were to live forever.” From: sonali dhindwal