Hi Pascal,
this behavior doesn't surprise me.
We had crystals in FC12, and didn't get any phase transitions in any
PEGs from 200 to 20.000, and with or w/o organic solvents such as MPD.
And I can tell you we had a lot of detergent with the purified protein,
concentrating on 50kDa.
With
Dear CCP4 Community,
We are currently looking for a postdoctoral fellow to join our research
programme on the regulation and functions of ADP-ribosyltransferases. Please
see the job description for the position and instructions on how to apply below.
Best wishes,
Sebastian
Post-doctoral
Pascal,
The reason this phenomenon looks odd to you is that detergent phase separation
is not a micelle size phenomenon, but a micelle surface phenomenon. Like any
colloidal solution, even protein, there can be conditions where the colloidal
particles aggregate (the cloud point), creating a
Dear all,
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Hi:
Despite Google and several attempts, I am still having no success in
getting Refmac5 to link a modified amino acid to its adjacent residues.
Using sketcher, a cif file for the modified amino acid was generated.
This cif file was used an input to Refmac5.
The relevant part from the Refmac5
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Dear all,
I'm refining a neutron structure and I would like to know whether there are
available geometry dictionaries for deuterated aminoacids, in order to
input them into Coot. At least the version I have (Coot 0.7, revision 4459)
is unable to handle aminoacids whose exchangeable H atoms are
Dear Gabriel (and the other members interested in how the issue was
solved; original post below):
You are this week's winner!! For getting the correct answer - it was
just a matter of editing the cif file and exchanging 'non-polymer' with
'peptide' - you get fame and glory on the CCP4 list,
For REFMAC, I think you need to alter the cif file for the modified residue -
such that the residue type is L-peptide rather than anything else.
Tony.
Sent from my iPhone
On 2 Aug 2013, at 17:08, Arnon Lavie la...@uic.edumailto:la...@uic.edu
wrote:
Hi:
Despite Google and several attempts, I
Hi Javier,
The deuterium exchange should not change the chemistry of the residues
(neglecting the minor changes in bond lengths), so the cif-file should be
exactly the same for the deuterated amino acids. The _chem_comp_atom.atom_id
(e.g. HA) in your monomer library (which is also a
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