I hesitated to post this because crystallography is meat and drink to people
around here, but in case it might be useful for teaching, here is the video of
a lecture I gave on X-ray crystallography at the Royal Institution on Friday
25th October. Warning: it lasts a full hour!
Dear Stephen,
Many thanks for this post.
The lecture is really great, I would recommended it to all interested in the
field (from beginners to seniors).
Best,
Isabel
Dr Isabel De Moraes, MRSC
Membrane Protein Laboratory
I've just tried to use LSQKAB to transform an entire trajectory file that is a
pdb file with ENDMDL cards between models to a new orientation for the purpose
of making use of work with the new orientation.
LSQKAB returns with an error:
*** RWBROOK error: point code unitfunction
***
Hi Charlie, one possible way of doing this is through UCSF-Chimera. It'll read each of the models into a different slot, then you can apply the transformation you choose to each of the models. I think I'm right. The Chimera support team may help you figure
out how to do this in one go.
Best
But at higher resolution you would like to release the restraints and introduce
more parameters in refinement, such as anisotropic Bs - ideally keeping the
observation/parameter ration more or less constant...
On 3 Nov 2013, at 12:50, Edward Berry wrote:
Looking at it from the other side-
Agreed. I was not entirely serious but just trying to present a diametrically opposed
viewpoint. A middle of the road approach would be to say the additional reflections could
benefit either refinement or ML (and maybe the two are not really separate since
ML guides the refinement), and so
Dear all,
Recently, I received the comments from referees, they asked for the SA-omit
map of the ssDNA of our protein-DNA complex. They said that simulated annealing
omit map better than a biased 2Fo-Fc. The ssDNA consists of seven thymidine
nucleotide. Our data diffracted to 2.65A,but the
