http://www.theguardian.com/commentisfree/2014/jan/02/in-praise-of-crystallography-editorial
P
Hi,
I'm working on DNA binding protein, looking to co-crystallize protein- DNA
complex and have no previous experience. Your suggestion would be very
precious on the following queries.
1. My protein is 646 amino acid long and it exists as homodimer. It is also
having around 20 amino acid
Dear Venkat,
We recently solved a protein DNA complex structure and I had all the same
queries as you have at the outset. We decided to use SPR as a techniques to
study the interaction of the protein with the DNA and to use this to guide the
choice of DNA for co-crystallisation. The work has
Save the date: ICCBM15, Sep. 17-20, Hamburg,
Germany
15th International Conference on the Crystallization of
Biological Macromolecules (http://www.iccbm15.org)
Focus on crystallization for novel radiation sources and cryo
Dear All,
For the question, I think for a protein-DNA interaction, the protein may
interact with any sequences of DNA, which will give a lot of combination of
protein-DNA sequence for crystallization screening. Or do anyone regard to
just try the crystallization of the protein-one specific
Dear All,
Thank you very much for your valuable suggestions.
Venkat
On Sat, Jan 4, 2014 at 9:53 AM, Acoot Brett acootbr...@yahoo.com wrote:
Dear All,
For the question, I think for a protein-DNA interaction, the protein may
interact with any sequences of DNA, which will give a lot of
