[ccp4bb] A question on protein-DNA complex crystallization

2014-01-09 Thread Acoot Brett
Dear All,   I am now working on the crystallization of a complex of protein-16 bp DNA by co-crystallization. In the screening very small needle-like crystal occurs. If not salt crystal, is there a method to know it is not the crystal of the DNA?   Cheers,   Acoot

Re: [ccp4bb] A question on protein-DNA complex crystallization

2014-01-09 Thread Raji Edayathumangalam
Hello There! Here are a couple of quick thoughts in response to your question, none of which will fully resolve your concern: (1) Wash your crystals, crush them, add native gel loading dye and run a native gel. First stain with ethidium bromide, then stain with coomassie blue. If you stain for