Re: [ccp4bb] XDS orientation matrix inconsistent with Mosflm, DENZO

2014-12-29 Thread Kay Diederichs
On Sun, 28 Dec 2014 15:37:22 -0600, Igor Petrik petr...@illinois.edu wrote: ... DIRECT BEAM COORDINATES (REC. ANGSTROEM) 0.003134 0.005401 1.020962 DETECTOR COORDINATES (PIXELS) OF DIRECT BEAM1230.87 1260.93 DETECTOR ORIGIN (PIXELS) AT 1226.25 1252.96 CRYSTAL

[ccp4bb] How far does rad dam travel?

2014-12-29 Thread Mohamed Noor
Dear all In a metal-containing crystal of (say) 200 um x 200 um, and a beam size of 10 um x 10 um, how far will I need to move away from an irradiated part to a fresh part to obtain an undamaged dataset? Exposure conditions: 100 % transmission at 10^12 ph/s, 0.1 s exposure, fine sliced at 0.1

Re: [ccp4bb] XDS orientation matrix inconsistent with Mosflm, DENZO

2014-12-29 Thread James Holton
Yes, unfortunately an orientation matrix is completely useless without a frame of reference. All it does is tell you where the cell axes lie relative to X, Y and Z, but X may be the x-ray beam, or sometimes that is Z and X is the spindle axis. Different people have different and strongly-held

[ccp4bb] Sokalan CP5 and CP7

2014-12-29 Thread Srinivasan Rengachari
Dear all,     I recently had a few initial hits for my protein in conditions with Sokalan CP5 and CP7 (Midas Screen). There is not much on the web about these compounds except for a thread on CCP4bb last year which doesn't include information about the suppliers.  The standard suppliers

Re: [ccp4bb] Sokalan CP5 and CP7

2014-12-29 Thread Pius Padayatti
you may buy them from BASF Even sometime back they gave some free samples for experimentation Call them directly Padayatti On Mon, Dec 29, 2014 at 2:59 PM, Srinivasan Rengachari 02062d79acb8-dmarc-requ...@jiscmail.ac.uk wrote: Dear all, I recently had a few initial hits for my