Dear all, I am working on a His-tag recombinant protein with two domains, which I purify using affinity chromatography. When I set up crystallization of the same, it gave me crystals in two different conditions- one was the complete protein. The other just had the Domain2.
Even on SDS-PAGE, I see three different bands of the purified protein. How can I determine the autu-proteolysis of this protein? This class of protein has not reported to have any auto-proteolytic properties, though it forms isopeptide bonds by autocatalysis. Thanks a lot Mohammad
