Hi Alex,
You may also think of gradually increasing PEG3350 concentration to around
40% which has worked pretty well in some of my cases.
Greetings,
Abhi
Abhimanyu Kumar Singh, Ph.D.
School of Biosciences
University of Kent
Canterbury, UK.
On 27 Sep 2016 23:43, "Alex Lee"
Probably you can not use thiocyanate as cryoprotectant because its a chaotropic
agent. Although its a very mild denaturant (destabilizer) but at high
concentration, it can destroy your crystals. I have recently used 30 % sucrose
for cryo protection of crystals growing under same condition and
Hi CCP4bb members,
I have a condition for my protein crystals at "0.2M sodium thiocyanate, 25%
PEG3350, pH6.9".
I'd like to try cryoprotectant with just higher concentration of sodium
thiocyanate, but I have no google hits of the range of concentration I
should use.
I wonder anyone in this
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