Thank you for the reply.
On Thu, Jan 26, 2017 at 9:07 PM, wrote:
> Dear Pooja,
>
>
>
> A few remarks:
>
>
>
> -Matthews does not show 4 chains in the asymmetric unit, it
> suggests 4 chains. However, in reality it can be more or less chains,
> although rare,
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* Extensive experience in enzyme kinetics studies,
Dear Loes,
thank you very much for your explanation. As agreed I hereby share the
conclusive email of your off-list discussion with John Helliwell, and a
pointer to your article with its explanation
http://dx.doi.org/10.1107/S1399004715011803:
Dear All,
thanks for your replies. I tend to believe that it could be ATP some
how copurified with the protein. Refinement with ATP, ADP+acetate, did
not vary too much in the B factors. The main concern is, only one xtal
was observed at this state ( mimicked like a ATP bound state and