>
> I know space is cheap these days, but is there a reason for Refmac to
> generate all those extra columns in the output mtz file? Refmac (as well
> as phenix.refine and buster-tnt) output mtz file is almost always used for
> only one purpose - look at the map in coot. You only need 4 columns
Hello everyone.
I remember the screen was again from Jenabioscience and this had happened
with one of my protein. The screen was very old and the condition was
peg3350, tris pH 8, lithium sulfate and NaCl as the salt. Hit was obtained
which was never reproducible. Luckily I solved the structure
?Hi Jonathan,
Hopefully you know about the trick of making any precious condition last longer
- use the 'magic' solution only in the drop itself, and use the best
approximation you can make in the reservoir of the experiment (if you are doing
vapour diffusion)
Regards, Janet
Janet Newman
Hi Jonathan,
Old buffer may have slow evaporation with some side reactions. The
concentration of each component may increase a little bit. In this case,
I would consider the condition as the origin for further optimization.
Each component may need to be considered separately.
For 150mM Tris
Perhaps a good opportunity for getting rid of (scaled) F and SIGF too?
Certain pipelines need Refmac's phase estimates (Buccaneer and Crank2 off
the top of my head), but I can't see how activating an 'expert mode' or
'developer mode' in order to get them would be a problem for their authors.
I know space is cheap these days, but is there a reason for Refmac to
generate all those extra columns in the output mtz file? Refmac (as well
as phenix.refine and buster-tnt) output mtz file is almost always used for
only one purpose - look at the map in coot. You only need 4 columns for
that,
Jonathan,
While your claim of oxidative degradation of PEG1000 may be true -- I gather
you mean that the conversion of the ends of the PEG polymers to aldehydes or
peroxides, then to carboxylates -- you should check out Fran Jurnak’s old
paper (Journal of Crystal Growth 76, 577, 1986). The
Hello,
Such pH drifts are rather common with crystallisation screens. Have you tried
to produce other precipitant solutions with ca. 47% PEG 1000, 80 mM Potassium
bromide but having a pH of (say) 6.2 ? A pH rangle close to that where your
crystals were obtained. This would mean changing
Dear CCP4bb community
I apologies for the slightly off topic post.
We have recently had success crystallizing a membrane protein (diffraction
> 3 Å at a synchrotron source) using the *in meso* method, the hit
condition was from the Jena Bioscience screen Pi-minimal condition number
#57.
Hit
Dear Shijun
As the message suggests, AMPLE has not been able to find the executable
of THESEUS, the program it uses for maximum likelihood superposition of
structures. This error message is nothing to do with Rosetta. THESEUS is
distributed with more recent versions of CCP4. To continue with
Hi all
It always fail when I run ccp4-AMPLE, and log file says
Cannot find executable theseus in PATH. Please give the path to theseus
***
* Information from CCP4Interface script
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