Dear Andy,
We published a paper a while back showing that we could use up to 2M urea to
prevent unwanted aggregation, and crystallize the sparingly soluble protein
(Dines et al. J Struct. Biol. 2007) and then showed that we could solve the
structure which was not affected by the presence of the
Dear All,
I have a protein from which I'd like to lose the co-purifying ligand (Coenzyme
A). The protein is his-tagged. The plan here is to bind it to a resin and then
run over a mild denaturant solution to encourage ligand loss (it seems that
extended dialysis does not achieve this).
Our star
You could always switch to a diaphragm vacuum pump - which is oil free -
and shouldn't be too fussed about working in a cold room. Quieter and
cleaner too.
Best wishes,
On 28 October 2017 at 18:17, Denis Rousseau
wrote:
> Does anyone have experience with a vacuum pump in cold room? We have been
Dear community,
sorry for a question non related to crystallography but I do not know where
to find relevant experts.
Please, look at the attached image:
In bacterial genome two operons, e.g. OPERON 1 and OPERON 2 lie close to
each other on different strands of DNA. Genes inside each operon have th
Hi,
If for any usual random reason you got to stick to this pump, try it out first
to see if it works, failing that talk to manufacturer and maybe change the oil,
failing that get a heating blanket to start it up, failing that use a dry pump
(checking if it likes operating at 4degC).
Cost the
Dear Robbie,
Thanks for posting that! When I share my slides, I generally try to do so only
as PDF files to make it harder for anyone to reuse them without permission, but
I hadn’t realised how much of an issue this is. It seems that I’ve nonetheless
let one PowerPoint into the wild (“Using m
Dear CCP4BBers,
Yesterday I ran into one of my talks at Slideplayer.com, a slide aggregator
that makes money from advertising. You can watch presentations for free
(surrounded by ads), but to download them you have advertise for Slideplayer by
posting on social media.
The presentations are seem
