Description:
A postdoctoral research associate position in membrane protein structural
biology/biochemistry is immediately available in the Department of Biochemistry
& Molecular Biology at Michigan State University, East Lansing, MI, US. One
major direction in Dr. Jian Hu’s laboratory is struc
On Thursday, 09 August 2018 10:45:07 Pavel Afonine wrote:
> > I (personally) think the best answer from these was to look at the
> > TLS-subtracted residuals (ie. total B-factor - TLS component) — can’t
> > remember who sent it, off the top of my head.
> >
>
> TLS is just an approximation, sometim
> I (personally) think the best answer from these was to look at the
> TLS-subtracted residuals (ie. total B-factor - TLS component) — can’t
> remember who sent it, off the top of my head.
>
TLS is just an approximation, sometimes good and sometimes not. If TLS
parameters are refined along with in
Hi,
A quick search of the CCP4BB archive reveals a couple of previous conversations
on this topic. I’ll link them here to save repetition — reading through should
give you a pretty good idea:
https://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg42526.html
https://www.mail-archive.com/ccp4bb@ji
I wouldnt have thought so.
There is a B factor plot as part of CCP4I2 - does that show any pattern of
differences between ligand and environ,ment?
Eleanor
On 9 August 2018 at 10:54, Santhosh Gatreddy
wrote:
> Hi all,
>
> I have to compare the B-factors of three of my ligand bound structures of
Well that is pretty obviously P 2 21 2 - h 0 0 and l 0 0 are obviously
present..
The 0 k 0 absences could be generated by a nc translation of x, 1/2, z - do
you have that?
Twinning is unusual in P2/mmm but possible of course - can you send the
pointless log file?
Eleanor
On 9 August 2018 at
Hi all,
I have to compare the B-factors of three of my ligand bound structures of the
same protein which were diffracted to 1.85, 1.98 and 2.01 A resolution.
Is it necessary to normalize the B-factors of these structures (dimer in ASU)
before comparing them to understand the ligand induced stab
Hi Tim - There are reflections in all directions and only one clearly has
systematic absenses. Although only few seem to be present in one direction (But
thats the h00 direction here
+ all are present though) -phaser finds with same data P22121 as the strongest
solution (few reflections are weak
Dear Tommi,
did you check whether you collected any reflections at all that should
be absent for the second screw axis? If there are non - which could
easily happen with low resolution, incomplete data - pointless and XDS
might be conservative and not estimate the likelihood for the second
screw-a
