In my case what simply worked is to put your solution with 40% glycerol on the
side of the drop and wait for crystals to stop dancing. Fish them out swiping
through the side were you have put your solution. Or I have used MPD as
cryoprotectant and freeze them in cold room (4C) its amazing what
Ahh, yes, the "dancing crystals problem". The good news is alcohols are
really good cryoprotectants as well as excellent precipitants. Shame
their use has fallen out of favor over the years, but I guess as drop
sizes got smaller the evaporation problems got worse and worse.
In my humble
Dear Colleagues
The Department of Biochemistry & Molecular Biology at the Indiana University
School of Medicine is seeking applications for tenure-track faculty positions
at the Assistant/Associate/Full Professor level positions in the area of
structural biology broadly defined as experimental
