This can be done at Diamond I23 beamline.
https://doi.org/10.1016/j.nimb.2016.12.005
Best regards,
Jan
Dne 16.02.2019 v 18:49 Patrick Loll napsal(a):
> S has about 0.56 anomalous electrons at 8 keV, whereas P has about
> 0.44. This is a small difference between two weak signals—unlikely to
>
Anomalous is the way to go but it helps to think about the final
concentrations used. With 50 mM PO4 (buffer) and 2 M SO4 (ammonium
sulfate) most "bindin sites in quesiton" will be occupied by SO4...
My 2 cents
Jeroen
On Sat, Feb 16, 2019 at 4:07 AM 张士军 <21620150150...@stu.xmu.edu.cn
S has about 0.56 anomalous electrons at 8 keV, whereas P has about 0.44. This
is a small difference between two weak signals—unlikely to give a clear result.
If you could get to the sulfur & phosphorus edges, then you could (in
principle) answer this, but that’s a very hard experiment to
Hi All,
I installed the latest ccp4 update on my MacBook through the CCP4i2 update
manager. After doing so, refmac appeared to be broken. I tried to restart the
I2 GUI but it refused to even after a reboot.
Eventually I got running again by uninstalling this update through the CCP4i
update
Two things to look at that could provide a clue:
Examine the anomalous map for some density over the central atom. Sulfur
will often, but not always have significant anomalous density depending on
the wavelength and quality of data set.
Phosphate is normally HPO4= or H2PO4-. Look for phosphate
How about collect data at sulfur peak. You might see anomalous peak for
sulfur.
Jinyu
On Sat, Feb 16, 2019 at 4:07 AM 张士军 <21620150150...@stu.xmu.edu.cn> wrote:
> Dear all
>
> I have got a crystal grown at the condition both have ion of SO4 and PO4,
> and the diffraction resolution is very
Dear all
I have got a crystal grown at the condition both have ion of SO4 and PO4, and
the diffraction resolution is very well, but the problem is coming: how to tell
which is which just from electron density? I think they are exactly same.
Thanks a lot !!!
Beat Regards
Shijun
