Re: [ccp4bb] Does ncs bias R-free? And if so, can it be avoided by special selection of the free set?

2019-06-02 Thread Eleanor Dodson
The current Rfree selection is done in the highest possible Laue group - eg trigonal uses P6/mmm - then the selection is proogated to the chosen Laue group - eg P3. So IF the ncs reflects a higher Laue symmetry as it often does the FreeR is sort of buffered against the ncs- effect.. That wont

Re: [ccp4bb] tNCS incompatible with cell dimensions

2019-06-02 Thread Eleanor Dodson
Hmm - that sym op means you have a near C centred cell with spacegroup C 2 2 2 ? Maybe some of your protein has been chewed up? That does happen? How good is the diffraction? Eleanor On Sat, 1 Jun 2019 at 17:44, Jonathan Cooper < 0c2488af9525-dmarc-requ...@jiscmail.ac.uk> wrote: > Does