Posted on behalf of Gert Vriend:
This article didn't make it to Nature Methods (...), but might be of interest
to theoreticians interested in B-factor distributions:
https://journals.calstate.edu/pump/article/view/2409/2168
Gert Vriend
Hi,
I agree with Rafael. Using of glycerol for aggregating proteins works
usually. Try also purifying in range of buffers (of course considering the
pI of the protein). A lower temperature may reduce the yield but worth a
try if it is aggregating at higher concentration.
You can also try
