Re: [ccp4bb] Co crystalization with less soluble ligand.

2021-06-23 Thread Tim Gruene
Hi Frank, heme becomes near completely insoluble when it dimerizes. Fatty acids are also very insoluble. It is quite hard to get them into HSA, as far as I remember. Cheers, Tim On Wed, 23 Jun 2021 10:33:42 +0100 Frank von Delft wrote: > And then of course, you need to decide whether you at

Re: [ccp4bb] Co crystalization with less soluble ligand.

2021-06-23 Thread Frank von Delft
And then of course, you need to decide whether you at all care to know anything about a compound that is so insoluble that it needs that kind of treatment :) On 23/06/2021 09:52, hoh wrote: Hi As total insolubility does not exist, I regularly use another method, which is to deposit a

[ccp4bb] Co crystalization with less soluble ligand.

2021-06-23 Thread hoh
Hi As total insolubility does not exist, I regularly use another method, which is to deposit a grain of the ligand directly into the drop. In this case, we no longer control the concentration. The goal is to regularly freeze crystals (1 hour, 2 days, 1 week ..). Depending on the results, it