[ccp4bb] Funded postdoctoral position (2+2 yrs) in Lund, Sweden

Dear Colleagues, Please forward the following announcement to potential candidates. Postdoctoral stipend in biochemistry and structural biology (Ref. 2021/2150) Lund University, Faculty of Science, Department of Chemistry, Center for Molecular Protein Science (CMPS) The city of Lund is situated

Re: [ccp4bb] Strange indexing problem

Dear Rob, at 1.5A resolution, you can comfortably run shelxe. If there is some useful phase information in your solution, this should complete your model, remove model bias and the resulting composition might tell you the proper space group. Rename your PDB-file into 'mymodel.pda' (pda, not pdb),

Re: [ccp4bb] AW: Strange indexing problem

Your self rotation strongly suggests a different spacegroup - I would look back at the integration and processing before puzzling over cell volumes! Eleanor On Mon, 5 Jul 2021 at 16:00, Schreuder, Herman /DE < herman.schreu...@sanofi.com> wrote: > Rob, > > > > Wat is the Matthews number, would i

Re: [ccp4bb] Strange indexing problem

Hi Rob, There is no information provided to think what might be the problem with indexing. How confident are you in the direct beam coordinates? Are any of the cell lengths long-ish? It may not be enough but at a minimum, two images 90 degrees apart might be helpful. Preferably, they would be taken

[ccp4bb] AW: Strange indexing problem

Rob, Wat is the Matthews number, would it fit with a very low percentage solvent, or would it not fit at all? What happens if you superimpose a tetramer on your dimer? Best, Herman Von: Robert S Phillips Gesendet: Montag, 5. Juli 2021 16:32 An: Schreuder, Herman /DE Betreff: Re: Strange inde

Re: [ccp4bb] Strange indexing problem

Hello, yes, the difference map looks great. If this one diffracts to near-atomic resolution, maybe you have a tetramer with a very low solvent content. Cheers, Jon.C.. Sent from ProtonMail mobile Original Message On 5 Jul 2021, 14:54, Robert S Phillips wrote: > I collected da

[ccp4bb] AW: Strange indexing problem

Dear Rob, Your screen shot shows a large empty region with difference density for the second half of the tetramer, so the my guess is that you have a tetramer in the asymmetric unit, but that your molrep program only found a dimer. Would a tetramer fit in the asymmetric unit? If you have a tetr

Re: [ccp4bb] Strange indexing problem

Why you have this problem I cannot guess, but your self-rotation clearly shows 2 2 2 symmetry. Try another data processing system? Eleanor On Mon, 5 Jul 2021 at 14:54, Robert S Phillips wrote: > I collected data last week on crystals of tyrosine phenol-lyase obtained > under new conditions. Th

[ccp4bb] PhD position in Vienna, Austria (University of Natural Resources and Life Sciences)

Dear colleagues, there is an opening for a PhD position in the Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria. The work will have a strong focus on structural biology methods, investigating a highly important enzyme involved in prokaryotic heme biosy

[ccp4bb] Open postdoctoral researcher position in Structural biology at University of Oulu, Finland

Dear All, We now have an open position for a *Postdoctoral Researcher* (fixed term for 2 years) to join our research group in the Protein and Structural Biology Research Unit, at the Faculty of Biochemistry and Molecular Medicine, University of Oulu, i

[ccp4bb] Bioinformatician position at PDBe - in collaboration with groups at UCL and Uni. of Washington

Dear Colleagues, We are looking for a bioinformatician/scientific programmer to join the Protein Data Bank in Europe (PDBe) team, working on a research project exploring the utility of residue covariation data in predicting protein quaternary structure. The project is a collaboration between PD

Re: [ccp4bb] Arcimboldo

Hi All Thank You very much for the response and suggestions. I have used Robetta and ITASSER for structure prediction followed by truncating the part according to the rmserror or loop region. However, I was not able to go anywhere after a certain point. R-free is stuck at 40. Even the loop region