Hi
Please note the closing date for applications for this post at Diamond is this
coming Sunday (12th November).
Best wishes
Dave
From: Hall, Dave (DLSLtd,RAL,LSCI)
Date: Monday, 23 October 2023 at 10:02
To: ccp4bb
Subject: Beamline Scientist position at Diamond Light Source
Hi Everyone
Shameless plug, did you try Fusion screen (available from MD). It has been
outperforming all the other 22 screens in our bank, either giving exclusive
hits or always giving crystals when other screens did too (sure we still had
cases when nothing work).
It has shown to be very powerful in our
... and reveal by WB if the protein is tagged.
The trickiest part of all this will be to rinse the "xtal(s)" as extensively as
possible in a protein solution before dissolving it for SDS-PAGE/WB or
MS or whichever method you select, in order to avoid contamination by the
protein-DNA complex
You could also run them on a gel if you don’t want to shot with canons on small
birds.
Jürgen
> On Nov 8, 2023, at 11:33 AM, M T wrote:
>
> Dear Careina,
>
> If you have easy access to mass spectrometry, you can try to fish/rince your
> « pumpkin seeds » and send them to mass to try to
Along the lines of mass spec analysis, but probably more accessible to
many, is gel electrophoresis. Rinsing enough crystals and dissolving them
in your protein buffer should give you a sample suitable for standard
agarose and polyacrylamide gel electrophoresis protocols. I showed that
crystals I
Dear Careina,
If you have easy access to mass spectrometry, you can try to fish/rince your «
pumpkin seeds » and send them to mass to try to identify what is inside to see
if it needs optimization or not.
Best.
> Le 8 nov. 2023 à 16:03, 02531c126adf-dmarc-requ...@jiscmail.ac.uk a écrit
>
Hi everyone,
I am looking for an experienced protein engineer to join my group.
Please, apply on the website, but also send me a copy of your CV.
Senior Scientist (Structural Biology) in Natick, Massachusetts | Careers at
Modex Therapeutics
Hi everyone,
I am looking for an experienced protein engineer to join my group.
Please, apply on the website, but also send me a copy of your CV.
Senior Scientist (Structural Biology) in Natick, Massachusetts | Careers at
Modex Therapeutics
Could be dna crystals. They often do not diffract.
Artem
On Wed, Nov 8, 2023, 11:18 AM careinaedgo...@yahoo.com <
02531c126adf-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> The reservior solution is 0.2 M NaCl2, 0.1 M HEPES pH 7.5, and 25% 3350 PEG
>
> Protein buffer is 300 mM NaCl, 20 mM HEPES
The reservior solution is 0.2 M NaCl2, 0.1 M HEPES pH 7.5, and 25% 3350 PEG
Protein buffer is 300 mM NaCl, 20 mM HEPES pH 7.5, 3mM TCEP
The drop contains 1.5ul protein-DNA complex and 1.5 ul reservior solution
On Wednesday, November 8, 2023 at 05:12:25 PM GMT+2,
Dear Careina
These look like crystals indeed. Without more information, I can make some
educated guesses but the ultimate proof is in your hands :)
1. they don't have to be spherulites. Note that low-symmetry crystals can
have edge-less habits, especially P1 are notorious for that - I've had
Hi Careina,
One thing I'd mention in addition to the spot-on replies so far is that
cryoprotection strategies can influence diffraction. These look like pretty
spherulites though, so best of luck in optimization!
Andrew
On Wed, Nov 8, 2023, 10:03 AM careinaedgo...@yahoo.com <
They look like a form of spherulites, which don't diffract as is but can likely
be optimized by matrix optimization, seeding, and such methods. Or in fact they
might just be crystals that need to be optimized into diffracting crystals.
Check this link for some ideas:
Have you tried crushing these up and seeding?
Jürgen
___
Jürgen Bosch, PhD, MBA
Center for Global Health & Diseases
Case Western Reserve University
Cleveland, OH 44106
https://www.linkedin.com/in/jubosch/
CEO & Co-Founder at InterRayBio, LLC
> On
Looks like classic, poorly-diffracting “potato” crystals to me.
Will need additional optimisation to convince them into a nicer morphology.
No diffraction indicates that they are likely not to be salt at least.
Tony.
Antony W Oliver FHEA, PhD
Principal Research Fellow
Genome Damage and
Hi Careina,
Without knowing what's in your protein buffer or crystallisation condition it's
hard to comment.
Best wishes,
Steve
Dr Stephen Carr
Research Complex at Harwell (RCaH)
Rutherford Appleton Laboratory
Harwell Oxford
Didcot
Oxon OX11 0FA
United Kingdom
Email
Hi allWe have been trying with no success to crystalize a protein. Recently we
got these strange shape "crystals". They are hard and flat but they do not
diffract at all. Any ideas as to what could cause this?Careina
To
Dear all,
Registration is open for the 2024 CCP4 study weekend entitled "Decision making
in MX - how to be a productive structural biologist".
This year we are focusing on decision-making in macromolecular crystallography:
when to use what automated tool, how to interpret the output and when
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