Re: [ccp4bb] Fragile Crystals

Dear Morgan Elizabeth, To complete the answers and suggestion made by the ccp4 community, I would add, that you can actually combine the service offered by HTX facility : You send your protein, and they are preparing the crystallization plate for you in CD-plate which may be use in both Harv

[ccp4bb] Serial Crystallography Workshop at Diamond Light Source

Dear all, We are pleased to announce that registration is now open for our 2024 Serial Crystallography Workshop at Diamond Light Source: Serial Crystallography Workshop 2024 - - Diamond Light Source The works

Re: [ccp4bb] CCP4 Study Weekend 2024 - In-person Early bird registration including standard student bursary CLOSES TODAY!

In-person early bird registration for the CCP4 Study Weekend 2024 closes today. Anyone registering for the in-person event after today will not be eligible for a standard student bursary and the price for standard delegates increases by £50. In-person registration will close on Monday 4th Decem

Re: [ccp4bb] Occupany refinement protocol amd best practices

Pavel has listed the papers that describe the underlying maths but unless you have high resolution data (> 1.9A say?) unscrambling results from occupancy plus B factor refinement is problematic to say the least.. Think about residues such as ARG or LYS where the termini are often extremely wobbly

Re: [ccp4bb] Fragile Crystals

Hi Morgan Elizabeth, You could try this protocol (paper title below, shameless plug), it can cryo-protect your crystals without the need of touching the crystal drop, while at the same time it is likely to make your crystals more robu

Re: [ccp4bb] Fragile Crystals

Hi Morgan, Cases like yours are the motivation why the PROXIMA 2A beamline (amongst other beamlines) have developed a plate screener for collecting X-ray diffraction data in situ. I suggest you contact those beamlines to find out how to get your plates in the beam. Cheers, Bill From: "Blak

Re: [ccp4bb] Fragile Crystals

Hi Well worth contacting your favourite (or favorite…) synchrotron about in-plate data collection - I’d imagine that APS has something in-place (there was a report way back in the depths of time [2015, when I still worked in the field…] about a prototype on ID-19?), and that might be easier to

[ccp4bb] Beamline scientist position at MicroMAX, MAX IV

Dear All, MAX IV Laboratory has an *open position for a beamline scientist in the MX group* to develop the user program at MicroMAX and drive beamline developments in the area of time-resolved serial crystallography together with the MicroMAX team and the MX group. Read more and apply here:

Re: [ccp4bb] Fragile Crystals

Dear Elizabeth, /In situ/ data collection is a good approach to try in your case. You could use the CrystalDirect technology for automated crystal harvesting that is more gentle to crystals than manual harvesting. This is  available both at EMBL Grenoble and EMBL Hamburg facilities, which off

[ccp4bb] Postdoc CNRS Bordeaux

Dear all, A Postdoctoral position is available in the laboratory of Nico Reyes at the CNRS in Bordeaux, France. We are an international team working on novel functional and pharmacological mechanisms of human membrane proteins. We combine functional and biophysical approaches (ITC, fluorescence