Dear all, thank you for the valuable inputs.
I realize that one important piece of information that must be added concerns
the relative orientation of the domain for which the electron density becomes
poor upon merging data towards higher symmetry, with respect to the rest of the
protein.
We
Dear all,
We are currently refining a low resolution model (3.9 A max), obtained by MR.
Dataset was collected from a single crystal with one long cell axis (~620 A)
and high solvent content (74%).
Best refinement results (by far!) are obtained in Refmac, by imposing P21 sg
(20 multidomain
Dear colleagues,
I intend to implement work on structural biology (by X-ray crystallography) of
membrane proteins in my lab, more specifically on the subset of inner
mitochondrial membrane carriers. For this, I would like to take this
opportunity and invite anybody who is prospecting a postdoc
Ahttp://www.ncbi.nlm.nih.gov/pubmed?term=Svatos%20A%5BAuthor%5Dcauthor=truecauthor_uid=11418499.
Chem Senses.http://www.ncbi.nlm.nih.gov/pubmed/11418499?dopt=Abstract 2001
Jun;26(5):529-31.
Uma.
--
Uma Katre
Columbus, IN 47201
From: Andre Luis Berteli Ambrosio
University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334Email:
rmgarav...@gmail.commailto:garav...@gmail.com
On Oct 3, 2013, at 8:42 AM, Andre Luis Berteli Ambrosio wrote
Hi,
We have been thinking of implementing recombinant protein expression in S.
cerevisiae in our lab, and I would like to ask if there is a choice of vector
designed for co-expression of any two intracellular binding partners, similarly
to the pETduet-1 vector for E. coli.
If you feel this is
Hi,
We have a case where a 260 KDa protein crystallized at 3 mg/ml, that was the
highest concentration we could achieve, without playing with the protein buffer.
Protein-to-well-solution ratio was 3:1.
When screening, we tested all ratios between 4:1 and 1:1. Crystals only
appeared at 3:1 ratio.
Dear ccp4bb,
I sometimes find the flux of x-ray sources reported in units of photons/s/0.1%
bandwidth instead of simply photons/s.
Where does the 1/0.1% bandwidth unit come from? I have also seen other
percentages like 0.01% bw or 0.02% bw...
Is it simply defining some degree of acceptance in
