[ccp4bb] ACA hotel room block closing June 17

Dear Colleagues, The ACA annual meeting in Portland is fast approaching. The meeting will be held from July 29 - August 3, 2022. The hotel room block at the Marriott Downtown Waterfront will be closing on June 17th. Book your room today ! R

[ccp4bb] Fwd: ACA2022: Travel Grant Application Deadline March 31, 2022

There is a travel award opportunity for students and postdocs to attend ACA 2022 in Portland, Oregon, July 29 - August 2, 2022. I encourage you to apply! The application deadline closes in two weeks. -Anna S. Gardberg, PhD ACA Meeting Committee Odyssey Pharmaceuticals -- Forwarded message

[ccp4bb] Industrial Structural Biology Position, Boston Area

Constellation’s Drug Discovery team is growing, and we are looking for a crystallographer to contribute to the structural characterization of epigenetic drug targets. This role will focus on recombinant protein construct design, wet-lab protein crystallization, and crystallography. More informatio

[ccp4bb] Some spots left for PSDI2014, detailed program now available

egards, The PSDI 2014 organizing committee: ·Tiago Bandeiras, IBET, Portugal ·Anna Gardberg, EMD Serono, USA ·Theresa Johnson, EMD Serono, USA ·Beate Matlok, Merck KGaA, Germany ·Djordje Musil, Merck KGaA, Germany psdi2...@merckgroup.com http://www.psdi2014.org

[ccp4bb] PSDI 2014, registration extended to September 30

d this announcement to anyone who may be interested in participating. We look forward to a great conference this fall. With kind regards, The PSDI 2014 organizing committee: ·Tiago Bandeiras, IBET, Portugal · Anna Gardberg, EMD Serono, USA ·Theresa Johnson, EMD Seron

[ccp4bb] PSDI 2014, Abstract and Registration Info

cement to anyone who may be interested in participating. We look forward to a great conference this fall. With kind regards, The PSDI 2014 organizing committee: ·Tiago Bandeiras, IBET, Portugal · Anna Gardberg, EMD Serono, USA ·Theresa Johnson, EMD Seron

Re: [ccp4bb] Ligand geometry obs. vs. ideal

I second Tim Gruene's suggestion of SHELXL; it's perfect for this. However, there is a steep learning curve, so I strongly urge you to pay close attention to the SHELXL (and SHELXPRO) manuals. On Wed, Sep 12, 2012 at 11:44 AM, Yuri Pompeu wrote: > Hi everyone, > I am trying to show that a ligand

Re: [ccp4bb] Off-topic: Best Scripting Language

Hi Jacob, As the preceding discussion has illustrated, there are obviously a number of options, nearly all of which will work well for what you describe. As Pete Meyer suggested above, the best language may be the one you already know (I also echo his suggestion to test your code on a small subset

Re: [ccp4bb] Suggestion for storage buffer for protein

Hi Christine, I'm just now catching up on some of your older posts, so this may be too late too help. For Fab crystallization, my successful protein stabilization buffer contained 20-50 mM MES pH 6.0 or 6.5 with the minimum possible amount of salt to stabilize the protein - 50 mM NaCl, perhaps. Low

Re: [ccp4bb] Optimizing xtals conditions

Hello Christine, I was working with Fab-peptide complexes and found that streak microseeding (for instance, with a cat whisker or horse-hair) was helpful for obtaining reproducible crystallization conditions - spontaneous nucleation relies on too many imponderables. However, I would also echo Yuri'

Re: [ccp4bb] cryo for high salt crystal

Hello, Min. Lithium sulfate is a cryoprotectant at 2.0 M and sometimes even less (much lower than the concentrations needed for ammonium sulfate), so I would try replacing your ammonium sulfate with lithium sulfate, creating a cryoprotectant with 0.5-1.0 M KCl, 1.4-2.0 M LiSO4, at pH 7. You might n

Re: [ccp4bb] Fab fragments and their stability

Hello Jan, I stored intact IgGs as an ammonium sulfate cut at 4C with a little azide for several months with no problems, but I can't recall ever trying long-term storage of the Fabs. Good luck. -Anna On Fri, Nov 6, 2009 at 8:52 AM, Jan Rash wrote: > Dear All, > > Does any body have any clue abo