I fully agree with Dima. We are able to co-express and purify two interacting
partners using pET28 and pET21 in E. coli. Some related references are :
J. Mol. Biol. (2011) 405,49–64
J. Biol. Chem. (2006) 281, 26491–26500
J Struct Biol. (2011) 175(2):159-70
Biswajit
- Original Message
in the expressed
protein. We would like to get this protein in heme-bound holo-form.
Any suggestion to trouble-shoot this problem would be highly appreciated.
Replies can be sent to me directly and I will post a summary on a later date.
Thanks in advance,
Sincerely yours,
Biswajit Pal
CCMB, Hyderabad, India
Hi Ivan,
Recently I used PatchDock (http://bioinfo3d.cs.tau.ac.il/PatchDock/index.html)
to find interface between two proteins.
Good luck,
Biswajit
Dr. Biswajit Pal, Scientist,
Centre for Cellular and Molecular Biology, E009,
Uppal Road, Hyderabad - 500 007, India.
Phone : +91-40-2719-2831,
Fax
