Re: [ccp4bb] Control the crystallization process in the presence of small volatile organic molecules

Hi Chen, I had a similar condition before (reservoir solution with 10% dioxane) and an oil-based cryoprotectant (66.5% w/w paraton-N, 28.5% paraffin oil and 5% glycerol) worked for me. Please check this paper out: http://scripts.iucr.org/cgi-bin/paper?wd5052 In addition, have you tried

[ccp4bb] Looking for open_EPMR

Thanks for your replies! Does anyone know where to find free open_EPMR software? It seems that website is no longer reachable. Chao

[ccp4bb] A dimer as a MR search model?

Dear CCP4 community: I am trying to solve a structure using Molecular Replacement. We obtained several crystal forms of the same molecule, which is a hetero-trimer(18KD+16kD+15kD). We have solved the structure of one crystal form(form_1), which has space group P 42 22, 1 molecule per

[ccp4bb] Help request: Failed MR using the same molecule as model

Dear CCP4 community: I am a beginner to crystallography and therefore my apologies if this question is too simple. Basically we obtained several crystal forms of the same molecule, which is a hetero- trimer containing protein A(18kD), protein B(16kD) and a RNA segment(40nt or about 15kD).

[ccp4bb] Help request: Failed MR using the same molecule as model

Dear CCP4 community: Sorry if there is a duplicate post. I am a beginner to crystallography and therefore my apologies if this question is too simple. Basically we obtained several crystal forms of the same molecule, which is a hetero- trimer containing protein A(18kD), protein B(16kD) and a