[ccp4bb] what is Potassium Sodium phosphate in wizard IIIIV screen

Dear all， I am using wizard screen and I have a condition of Potassium Sodium phosphate. what is Potassium Sodium phosphate in wizard V screen. Best regards, Weifei

[ccp4bb] DNA RNA annealing problem

Dear all, I want to get a complex of DNA-RNA-protein. But I have a big problem of annealing DNA-RNA. The length of DNA is 19nt and RNA is 17nt. Annealing protocol: 2uM DNA 2uM RNA 10mM Tris-Hcl 100mMNaCl 1mMEDTA Heated to 95 for 5min, cooling down slowly for nearly 2h to room temperature. I can

[ccp4bb] phenix installtion problem

Dear all, Sorry to disturb you. I have install Phenix to my computer Centos 7.0. When I want to use it I need to cd /usr/local/phenix-1.9-1692/ and . /usr/local/phenix-1.9-1692/phenix_env.sh then type phenix in the command line every time. I wish to use phenix in every terminate window and

[ccp4bb] What screens to buy

Dear all, I want to buy some screens for Protein-RNA complex. What do you recommend ? Sincerely， Weifei