Re: [ccp4bb] Any suggestions?

2017-07-07 Thread Dr. Isabel De Moraes
there are numerous possibilities. Kind regards, Isabel On 7 Jul 2017, at 12:57, Dr. Isabel De Moraes <isabel.de-mor...@diamond.ac.uk<mailto:isabel.de-mor...@diamond.ac.uk>> wrote: Any suggestions regarding to the positive densities? The crystallisation condition only has N

[ccp4bb] Any suggestions?

2017-07-07 Thread Dr. Isabel De Moraes
Any suggestions regarding to the positive densities? The crystallisation condition only has NaCl and CaCl [cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk] [cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk] Best regards, Isabel -- This e-mail and any attachments may

[ccp4bb] Research Technician for the Membrane Protein Laboratory (MPL) at Diamond Light Source and RCAH in Oxfordshire

2017-04-21 Thread Dr. Isabel De Moraes
Dear all, Applications are invited for a full-time Research Technician position at the Membrane Protein Laboratory (MPL) located at the Diamond Light Source and at the Research Complex (Oxfordshire). The MPL is Wellcome Trust funded facility created to allow scientists to determine

[ccp4bb] Research Assistant for the Membrane Protein Laboratory (MPL) at Diamond Light Source and RCAH in Oxfordshire

2017-04-21 Thread Dr. Isabel De Moraes
Dear all, Applications are invited for a full-time Research Assistant position at the Membrane Protein Laboratory (MPL) located at the Diamond Light Source and at the Research Complex (Oxfordshire). The MPL is Wellcome Trust funded facility created to allow scientists to determine medically

Re: [ccp4bb] Dimer in SDS-PAGE

2017-02-21 Thread Dr. Isabel De Moraes
Dear Amit, You are working with a membrane protein and the use of SDS (harsh detergent) often makes these proteins to oligomerise. Boiling your sample is not advisable, it might make it worse. I’m not sure why you would like to “see” a monomer in the gel but if you really would like to know

[ccp4bb] FREE Symposium on Membrane Protein Sample Preparation & Analysis

2016-10-07 Thread Dr. Isabel De Moraes
Dear CCP4 colleagues, The Membrane Protein Laboratory (MPL) at the Diamond Light Source and GE Healthcare are organising a FREE Symposium on Membrane Protein Sample Preparation & Analysis. The symposium will take place on the 10th November, 9:30am at the Pickvance Lecture Theatre, Harwell

Re: [ccp4bb] TM purification & crystallization

2016-07-28 Thread Dr. Isabel De Moraes
Dear Andre, It is a small protein then, I recommend to do the purification in a small micelle detergent (e.g. OG). This regardless the extraction done in DDM or DM or any other suitable detergent. You can start by reading Moraes, Isabel, et al. "Membrane protein structure determination—the

[ccp4bb] Position for a Research Technician at the Membrane Protein Laboratory at Diamond Light Source

2014-06-13 Thread Dr. Isabel De Moraes
/irc/candidateSelfService/webui/VisVacDispPGakRegionApplicationId=821transactionid=1538103408retainAM=YaddBreadCrumb=Sp_svid=43842p_spid=1669138oapc=9oas=dHtHWqciP0_3C_VtnV-LXQ Kind regards, Isabel Dr Isabel De Moraes, MRSC Membrane

Re: [ccp4bb] Royal Institution Lecture

2013-11-03 Thread Dr. Isabel De Moraes
Dear Stephen, Many thanks for this post. The lecture is really great, I would recommended it to all interested in the field (from beginners to seniors). Best, Isabel Dr Isabel De Moraes, MRSC Membrane Protein Laboratory

Re: [ccp4bb] membrane protein optimization

2013-10-23 Thread Dr. Isabel De Moraes
Dr Isabel De Moraes, MRSC Membrane Protein Laboratory Diamond Light Source Ltd, Harwell Science and Innovation Campus, Chilton, Didcot, Oxfordshire, OX11 ODE, UK On 23 Oct

[ccp4bb] PhD position at the Membrane Protein Laboratory (Diamond)

2013-05-10 Thread Dr. Isabel De Moraes
three years. For informal enquires please contact Dr Isabel Moraes (i.mor...@imperial.ac.ukmailto:i.mor...@imperial.ac.uk) Dr Isabel De Moraes, MRSC Head of the Membrane Protein Laboratory Diamond Light Source Ltd, Harwell Science

[ccp4bb] A FREE course in High-throughput Protein Production and Crystallisation

2013-01-09 Thread Dr. Isabel De Moraes
, at 15:52, Dr. Isabel De Moraes wrote: Dear Structural Biologists, The Oxford Protein Production Facility (OPPF-UK) and The Membrane Protein Laboratory (MPL) at Diamond are offering a hands-on course in High-throughput Protein Production and Crystallization. The course runs from 30th

Re: [ccp4bb] A FREE course in High-throughput Protein Production and Crystallisation

2013-01-09 Thread Dr. Isabel De Moraes
Dear All, I would like to retract this post since the information is wrong. My sincerely apologies. I will send the right information shortly. Isabel On 9 Jan 2013, at 11:16, Dr. Isabel De Moraes wrote: Dear All, A gentle reminder that the deadline for the practical course

[ccp4bb] EMBO Practical Course in “High throughput Protein Production and Crystallization

2013-01-09 Thread Dr. Isabel De Moraes
://events.embo.org/13-htp-protein/application.html With best Regards, Isabel On 14 Oct 2012, at 13:09, Dr. Isabel De Moraes wrote: Apologies for the previous email, almost impossible to read. Hopefully, this one looks better. Dear All, We are happy to announce an EMBO Practical Course

Re: [ccp4bb] Crystallography on BBC Radio 4 next week

2012-11-22 Thread Dr. Isabel De Moraes
Deal All, Like many others, I also would like to encourage to listen the “In Our Time” BBC Radio 4 program next week as part of the Bragg Centenary Celebration. Melvyn will have Prof Mike Glazer (Oxford), Dr Chris Hammond (Leeds) and Prof Judith Howard (Durham) as guests. A subject not to be

[ccp4bb] EMBO Practical Course in “High throughput Protein Production and Crystallization

2012-10-14 Thread Dr. Isabel De Moraes
Apologies for the previous email, almost impossible to read. Hopefully, this one looks better. Dear All, We are happy to announce an EMBO Practical Course in “High throughput Protein Production and Crystallisation” The overall aim of the course will be to review the state-of-the-art in

[ccp4bb] EMBO Practical Course in “High throughput Protein Production and Crystallization

2012-10-12 Thread Dr. Isabel De Moraes
Dear All, We are happy to announce an EMBO Practical Course in “High throughput Protein Production and Crystallization” The overall aim of the course will be to review the state-of-the-art in HTP structural biology with an emphasis on methods to study complex targets, including membrane

[ccp4bb] Symposium in Membrane Protein Crystallisation - UK

2012-08-10 Thread Dr. Isabel De Moraes
. With best Regards, Isabel Moraes - Dr. Isabel De Moraes, MRSC Membrane Protein Laboratory Facility Co-ordinator Membrane Protein Laboratory Diamond Light Source Ltd, Chilton, Didcot, Oxfordshire, OX11 ODE

Re: [ccp4bb] Detergent and protein oligomerization

2012-06-21 Thread Dr. Isabel De Moraes
Dear Raji, The best way to find out is to run a SEC-MALLS (Size Exclusion Chomatography - Multi-Angle Laser Light Scattering) experiment. Best, Isabel - Dr. Isabel De Moraes, MRSC Membrane Protein

[ccp4bb] Practical Membrane Protein Course in Brazil

2012-06-21 Thread Dr. Isabel De Moraes
, Isabel - Dr. Isabel De Moraes, MRSC Membrane Protein Laboratory Facility Co-ordinator Membrane Protein Laboratory Diamond Light Source Ltd, Chilton, Didcot, Oxfordshire, OX11 ODE, UK Tel (direct): 01235

Re: [ccp4bb] Automating liquid dispensing

2011-03-13 Thread Dr. Isabel De Moraes
between plates due to the tip washes). Regards, Isabel - Dr. Isabel De Moraes, MRSC Membrane Protein Laboratory Facilities Co-ordinator/Group Leader Membrane Protein Laboratory, Diamond Light Source Ltd, Harwell Science and Innovation Campus

Re: [ccp4bb] Liquid nitrogen resistant flooring

2011-02-16 Thread Dr. Isabel De Moraes
are around Diamond you are welcome to have a look. Regards, Isabel - Dr. Isabel De Moraes, MRSC Membrane Protein Laboratory Facilities Co-ordinator/Group Leader Membrane Protein Laboratory, Diamond Light Source Ltd, Harwell Science and Innovation Campus

[ccp4bb] Call for applications to the Membrane Protein Lab at Diamond

2010-10-08 Thread Dr. Isabel De Moraes
Call for applications to the Membrane Protein Lab at Diamond Dear Crystallographers, The Membrane Protein Laboratory (MPL) at Diamond Light Source in Oxfordshire (UK) is pleased to announce that is now open our call for proposals. The MPL is a joint venture between the Diamond