is interested/suitable
and eager to come to NZ we are keen to hear from you.
Thanks.
Jodie
--
Dr Jodie Johnston
Senior Lecturer
Department of Chemical and Physical Sciences
University of Canterbury
Christchurch
New Zealand
e-mail: jodie.johns...@canterbury.ac.nz
This email may be confidential
Hi Florian,
Not sure if this is helpful or not but I have a particular protein that seems
to be either P22121 (with 2 molecules) or I222 (with one molecule).
Not from the same drop but crystallised in similar conditions but with
different small molecules bound.
In many cases the crystals
Hi
I wanted to check is it possible that there could
be errors in the library cif files associated with CCP4 and coot ?
I have noticed a couple of potential errors in a ligand cif file from the CCP4
library.
Perhaps it is my error - I shall need to triple check it again but, if it
,
except for the matter if there is 'right' and 'wrong' in science or
rather 'good' and 'bad' models, Garib Murshudov maintains the
cif-library and if you send him a compilation of possible fixes he
would surely appreciate your input.
Best,
Tim
On 10/29/2013 10:15 AM, Jodie Johnston wrote:
Hi
Hi Theresa,
I am right in thinking the protein construct you are using is just 8 His
residues added to 90kDa ?
It could be (if this is the case) that the tag is not accessible for binding
to the Ni column. Sometimes you need a linker sequence between the protein for
the
His-tag to coordinate
