[ccp4bb] PDB-REDO question

find such a file for the deposited structures. Is there a way to get my hands on it? Thanks in advance & best wishes, Joern -- * Address: Joern Krausze Braunschweig University of Technology Institute of Plant Biology Spielmannstr. 7 38106 Bra

Re: [ccp4bb] Up-to-date 3D stereo solution

list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ -- * Address: Joern Krausze Department of Plant Biology Braunschweig University of Technology Spielmannstr. 7 38106 Braunschweig

[ccp4bb] Refmac5 question

Joern -- * Address: Joern Krausze Department of Plant Biology Braunschweig University of Technology Spielmannstr. 7 38106 Braunschweig Germany Email: j.krau...@tu-braunschweig.de Phone: +49 (0)531 39

Re: [ccp4bb] 3D

schul...@cornell.edu To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- * Address: J

Re: [ccp4bb] another unknown density problem

Dear all, I think Abhishek means that the picture shows 5 A map radius. Best Joern > On 3. Nov 2017, at 18:42, Pavel Afonine wrote: > > If by "it was truncated at 5A for clarity" you really mean you truncated all > low-resolution data from 5A and lower then I am not surprised you see funny >

Re: [ccp4bb] getting error while running Ample

Dear Yogesh, I mean no offense but did you read your log-files? They are quite obvious about what is wrong. Regards Joern Sent from my iPad > On 14.11.2014, at 18:07, yogesh khandokar wrote: > > Dear ALL > Many thanks to my previous query. Can you please help me to fix error while > runnin

Re: [ccp4bb] Room temperature data collection

Dear Theresa, We recently collected a room temperature data set from one single crystal at Petra III. The beam line was equipped with a Pilatus detector. Data were good to 2.7 A. In contrast, at 100 K similar crystals diffracted very poorly. So, it is perfectly possible to obtain useful room te

Re: [ccp4bb] Helix forming proteins crystallizing as rings

help. Best regards, Joern ** Address: Joern Krausze Molecular Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany Email: joern.krau...@helmholtz-hzi.de Phone: +49 (0)531 6181 7023 (office) +49 (0

[ccp4bb] Helix forming proteins crystallizing as rings

** Address: Joern Krausze Molecular Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany Email: joern.krau...@helmholtz-hzi.de Phone: +49 (0)531 6181 7023 (office) +49 (0)531 6181 7020 (lab

Re: [ccp4bb] Off Topic- Cystine Detection

thods should indicate the presence of disulfide bonds, but will probably not allow for their actual quantification. Regards, Joern ** Address: Joern Krausze Molecular Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunsc

Re: [ccp4bb] electron density assignment

certain beyond doubt is that it was somehow caused by the presence of cobalt ions. Do you, by chance, also have cobalt or similar ions present in your crystallization condition? Regards, Joern ** Address: Joern Krausze Molecular Structural Biology Helmholtz

Re: [ccp4bb] How to exclude resolution bins

Hi, in XDS this can be done quite easily. Just use the line EXCLUDE_RESOLUTION_RANGE=3.7 3.57 in the XDS.INP file. Joern ** Address: Joern Krausze Molecular Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124

Re: [ccp4bb] Puzzling electron density upon heavy metal soaking

Here is a snapshot of the anomalous map: http://imageshack.us/a/img560/1905/anomalousmap.png The map is contoured to 2.5sigma. They are the strongest peaks in the map. Not all 7 positions are equally strong. J. ** Address: Joern Krausze Molecular

Re: [ccp4bb] Puzzling electron density upon heavy metal soaking

all 7 position. J. ** Address: Joern Krausze Molecular Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany Email: joern.krau...@helmholtz-hzi.de Phone: +49 (0)531 6181 7023 (office) +49 (0)531 6181

Re: [ccp4bb] Puzzling electron density upon heavy metal soaking

, Joern Krausze wrote: Dear all, I have two isomorphous crystals of the same protein. One crystal, let's call it 'derivative', was soaked with 10 mM CoCl2 whereas the other, let's call it 'original', was not. Both crystals were otherwise grown under identical conditio

[ccp4bb] Puzzling electron density upon heavy metal soaking

Thank you in advance! Joern ********** Address: Joern Krausze Molecular Structural Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany Email: joern.krau...@helmholtz-hzi.de Phone: +49 (0)531 6181 7023 (office)

Re: [ccp4bb] reifine metal with phenix

resseq 113 distance_ideal = 2.1000 sigma = 0.01 } You can check the phenix hompage for detailed documentation: http://phenix-online.org/documentation/refinement.htm Yours, Joern ** Address: Joern Krausze University of Leipzig Centre for Biotech

Re: [ccp4bb] Co-crystallization

dent Duke University ** Address: Joern Krausze University of Leipzig Centre for Biotechnology and Biomedicine Deutscher Platz 5 04103 Leipzig Germany eMail: [EMAIL PROTECTED] Phone: +49 (0)341 9731312 Fax:+49 (0)341 9731319 **