Re: [ccp4bb] saturn 724 query

Hi, Does anyone know if a Rigaku Saturn 724 ccd can be used for protein crystal data collection or only for small molecule ? Thanks Magnus Alphey University of St. Andrews To unsubscribe from the CCP4BB list, click

Re: [ccp4bb] Preparation of seed-stocks without seed-beads

You can always make your own 'seed-bead' using a French Press nylon ball (1/8) and an eppendorf. Or in the absence of French Press consumables suppliers, just find your local industrial gadget supplier (I found Dejay Distribution Ltd in the UK via google). They quoted me £4.45 for 100 x 1/8 nylon

Re: [ccp4bb] Off topic: 182 kDa protein is too large to crystallize

Hi Marek, I agree with Tim. 182kDa is certainly not too large for crystallization. As for purification . Have you tried different buffer conditions for your Ni-NTA purification ? Adjusting the pH or salt component may improve the purification sufficiently. You could try changing the salt