[ccp4bb] Job opening at the Albany College of Pharmacy and Health Sciences

2017-10-06 Thread Manish Shah
Dear colleagues, Please forward the following open position in molecular and structural biology to potential candidates. https://employment.acphs.edu/postings/490 Thank you. Manish Manish B. Shah, Ph.D. Assistant Professor Department of Pharmaceutical Sciences Albany College of

[ccp4bb] Fwd: Structure with high B-factor

2015-03-17 Thread Manish Shah
Hi All, I solved a structure using molecular replacement (Phaser) and the current R-factor and R-free is 0.2 and 0.25. The electron density looks great for residues and the ligand, however, the issue is with the B-values. The average B-value is 110 at this stage of refinement and I could not

Re: [ccp4bb] An alternative to glycerol in keeping protein soluble?

2010-02-23 Thread Manish Shah
Use 0.5 M Sucrose in your buffer, replacing 10 or 20 % glycerol. It works well. Manish On Tue, Feb 23, 2010 at 3:07 AM, SERAH KIMANI serah.kim...@uct.ac.za wrote: Dear all, Does anyone have an idea of something else that I can use instead glycerol to maintain solubility of my protein? I