Hi all -
I am looking for examples of structures of protein-protein complexes
(homo or hetero) wherein the crystallized proteins had mutations
within their binding interfaces, and yet the same (weakened)
interactions were still recapitulated in crystal contacts.
Any leads would be much
Could anybody tell me how to detect the concentration of detergent?
From Butler et al. (2004) J Mol Biol 340: 797-808
The concentration of DDM was determined by a colorimetric assay that
detects the sugar component of the detergent, which has given results
identical with the standard
the tilt is fixed you should be able to figure out the
rotational translation in your second hexamer.
Enjoy your puzzle,
Jürgen
P.S. P3 is certain ? Check with pointless or by human brain visual
inspection (HBVI)
On May 15, 2010, at 11:53 PM, Owen Pornillos wrote:
Dear ccp4bb –
I have
Dear ccp4bb –
I have questions with regards to crystal disorder that gives rise to
translational pseudosymmetry.
We have a rotationally hexameric protein that crystallized in P3, with
one hexamer in the asu. The local 6-fold axis of the hexamer is non-
crystallographic, and is
