, and
proteases, to advance drug discovery, with the opportunity for superb
mentorship support from the programme, which involves the labs of Laura Itzhaki
(degraders; PMID 33623657), Paul Miller (ion channel antibodies; PMID
35140402), Florian Holfelder (proteases; PMID 36765057), Mark Howarth
Applications are invited for a postdoctoral researcher position in the
laboratory of Dr Paul Miller at the Department of Pharmacology, University of
Cambridge, beginning from January – April 2023.
The project will study small molecule and/or antibody modulators of ion
channels as research
Applications are invited for a postdoctoral researcher position in the
laboratory of Dr Paul Miller at the Department of Pharmacology, University of
Cambridge, beginning from around October 2022.
The project will focus on studying the molecular basis of antibody modulation
of ion channel
the laboratory of Dr Paul Miller at
the Department of Pharmacology, University of Cambridge, beginning from around
October 2020.
The project will focus on studying the molecular basis of antibody modulation
of ion channel function and applying structure-guided approaches to engineer
unique
Applications are invited for a postdoctoral (research associate) position
(18-months in the first instance) in the laboratory of Dr Paul Miller at the
Department of Pharmacology, University of Cambridge, beginning from 1st
November 2020.
The project will focus on studying the molecular basis
Absolutely you should optimise! It's impossible to predict crystal behaviour. I
had a 20 A crystal, and I set a new plate in the same reservoir with an
additive screen and got a 3A crystal. It was probably a completely different
crystal to be honest, lattice, etc, but one never knows, you just h
I had a similar problem to what you describe. In my case the dataset was
severely anisotropic (2.6A, 3.4A, 4.6A in each axis). The R factors were stuck
similar to yours but the map looked good. I was told by someone with a much
better appreciation of the theory than myself that the anisotropy wa
Only one of your dialysis buffers has a decent (100 mM) NaCl. Maybe you could
try higher salt IN COMBINATION with glycerol. There's also NDSBs that stabilise
proteins.
Also, could the high immidazole be keeping the protein happy? You could test
this by dialysing into the same buffer with a rang
