Hi Zhen,
I also had problems with low amounts of plasmid from yeast using 5 ml o/n
cultures. But when increasing the culture volume it worked:
Large-scale (0.51 l for obtaining preparative plasmid amounts) overnight
yeast cultures were harvested, washed in cold water once, and then
resuspended
Dear all,
I have a question concerning removal of a his-tag sequence.
We have crystallized a protein with an important feature at the C-terminal
part of the protein.
Unfortunately, we cannot express it with a N-terminal his-tag, only with a
C-terminal his-tag.
Therefore we are looking for a
Hi YB,
There is a nice new paper online, dealing with this subject:
http://www.ncbi.nlm.nih.gov/pubmed/20850411
Cheers
Phil
Am 04.10.10 17:09 schrieb Owen Pornillos unter o...@scripps.edu:
Could anybody tell me how to detect the concentration of detergent?
From Butler et al. (2004) J Mol
