[ccp4bb]

Ok, we just needed to renumber the reside and change HETATM to ATOM and the became linked to the main chain again. Thanks all *Renato Mateus Domingos* *PhD student at Departamento de Genética e Biologia Evolutiva* *Universidade de São Paulo, Brazil* *Phone: (+55)(11)30917590* On Tue, Oct 16

[ccp4bb]

way to make the proper bonds. Anyone can help? Renato *Renato Mateus Domingos* *PhD student at Departamento de Genética e Biologia Evolutiva* *Universidade de São Paulo, Brazil* *Phone: (+55)(11)30917590* To unsubscribe fro

[ccp4bb] pdb2pqr-electrostatic potential

to recognize CYM as unprotonated CYS and keep CYS protonated? Thanks Renato *Renato Mateus Domingos* *PhD student at Departamento de Genética e Biologia Evolutiva* *Universidade de São Paulo, Brazil* *Phone: (+55)(11)30917590*

Re: [ccp4bb] ADD sulfinic-cysteine to protein aa-chain

Thanks all for the help! Definitely, using: Extensions -> Modelling -> Replace Residue ... -> CSD -> Mutate was the easiest way to solve the problem of adding the sulfinic cysteine! Thanks Renato *Renato Mateus Domingos* *PhD student at Departamento de Genética e Biolog

[ccp4bb] ADD sulfinic-cysteine to protein aa-chain

Hi I add the sulfinic cisteine in my coordenate map, however I am strugling to make proper bonds between the aa chain of the protein and the sulfinic cysteine I added. the PDB does not recognize the modified cysteine I added as an amino acid. Any help please? thanks *Renato Mateus Domingos* *PhD