Re: [ccp4bb] is codon optimization worth it?

2011-09-30 Thread Torres-Larios Alfredo
 We recently published something really old-related about it: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021035 My two cents. Best, Alfredo. Alfredo Torres-Larios, PhD Assistant Professor Instituto de Fisiologia Celular, UNAM Mexico

[ccp4bb] Posttranslational modifications of recombinant proteins expressed in E. coli?

2009-01-19 Thread Torres-Larios Alfredo
Dear all, I know this sounds weird (and besides, it's a non CCP4 question), but does anyone know any reports or has worked with a recombinant protein that is apparently post translationally modified by E. coli? We have an enzyme with two populations that have been sequenced at the

[ccp4bb] UV light source for protein xtal detection

2008-05-14 Thread Torres-Larios Alfredo
Dear all, Here's another non CCP4 question: does anyone know a cheap alternative to set up a UV source at 280 nm? I'd really like to have one :), but I really don't have the $20K Dlls needed to buy a UV/white light source from the crystallographic vendors :(. Thanks so much in advance for