Re: [ccp4bb] determining the domain for overexpression and crystallization

2011-03-17 Thread Xianhui Wu
://scratch.proteomics.ics.uci.edu/ to have an idea of the intrinsically disordered regions in the protein, transmemebrane regions and disulfide bonds that would certainly help you in initiating in the right direction. Best wishes Gauri On Mon, Mar 7, 2011 at 4:10 AM, Xianhui Wu wuxian

[ccp4bb] determining the domain for overexpression and crystallization

2011-03-07 Thread Xianhui Wu
Dear all, Before we try to study the crystal structure of an unknown protein, we need to determine the sequence that can fold into a compact and stable 3D domain. What kinds of methods can we choose? -- Best regards, XH Wu

[ccp4bb] molecular replacement

2010-06-24 Thread Xianhui Wu
Dear All, I am trying to resolve a protein structure with the use of Molecular Replacement. However, some part of protein are overlap in the interface of homodimer. Would someone please give me suggestions? Thank you! -- Best regards, WuXH

Re: [ccp4bb] Unexplained density

2010-04-29 Thread Xianhui Wu
I think it could be zinc ion. On Fri, Apr 30, 2010 at 5:38 AM, Daniel Bonsor bon...@bbri.org wrote: Hello again I currently have some unexplained density in my structure. As you can hopefully see from the images (see file), the density is dumbbell shaped. Whatever it is, it is coordinated