[ccp4bb] DNA Restraints or Cif file for Phenix refinement

2013-03-19 Thread Yurong Wen
Dear All, Recently I generated a dataset of DNA protein complex. I built the DNA model with the simple mutate of nucleic acids, however when I tried to refine my structure, I came to the fatal error below with unknown bonded energy atom. (Similar to the phenix Tutorial 10: Generating ligand

[ccp4bb] Pseudo translational symmetry Problem or Wrong Spacegroup

2012-12-03 Thread Yurong Wen
Dear All, Recently, I collected a dataset of a protein-DNA complex and indexed in spacegroup P212121 to 3.4 Å. However, Phenix.Xtriage indicated the presence of a very high pseudotranslational symmetry peak. I scaled the data in spacegroup P222. When I used the protein heterotetramer as