[ccp4bb] protein forming dimers

Hello everyone, I would like to take a advice from you all. I have protein like 200 KDa when i lode in a gel it shows band on 200KDa, Is that this protein gets dimer or anything else is happening. I would appreciate for some open discussion. -- * Regards,** Arun *

[ccp4bb] about point mutation

can any one help me in suggesting that what mistake i have did in my mutagenic pcr . actually my problem is my primer annealing temperature is 81degree. im using phusion pol enzyme. i have made many trial, i.e., made annealing at 68 and then followed 2 step pcr method and then added 1micro lit