Hi,
In my experience, it is very very very difficult to optimize this needle like
crystal. Always give up.
Good luck!
Dear All;
Could somebody give a nice suggestion how the following type crystal could
be optimized, I almost tried everything.
Crystal Image is attached
Crystal condition:
Hi,
For me it is detergent crystals or other stuff that is not quite important in
this case. As you showed the resolution is quite low. I suggest you need to
repeat again again again. You may go to PEG200. In our case, it is very
difficult to reproduce membrane protein crystals.
Good luck!
Hi,
I used 0.017% or 0.012%. My protein is very stable at this concentration.
Good luck.
Lin
2012-03-26
yybbll
Hi All,
Has anyone had any luck purifying membrane proteins with DDM
(n-dodecyl-D-maltoside) using concentration of detergent ~1-2 x CMC ? (Its CMC
is very low: 0.009
Additives screening.
YB
2011-02-21
yybbll
发件人： Bosch, Juergen
发送时间： 2011-02-21 03:46:12
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] optimization of dumbbell like crystal
Seeding ?
changing from sitting to hanging drop or under oil ?
play with temperature ?
Jürgen
On Feb 20, 2011, at 9
Hi, thank you for you reply. Could you tell me if you try to dye membrane
protein crystal with detergent?
Thanks.
Y.B.
2010-11-18
yybbll
发件人： Matthew Bratkowski
发送时间： 2010-11-17 02:58:40
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using
the concentration of detergent?
And how to dilute detergent?
Thanks all.
Y.B. Lin
2010-10-04
yybbll
Hi,
A little danger this condition. I suggest you should firstly make sure they are
protein crystals. I know it is very difficult. I suggest you can add proteases
to the drops. If crystals dissolve, no problem, they are protein crystals.
Best
Yibin
2010-08-27
yybbll
发件人： rui
发送时间
Hi, Did you use detergent? It seems look like detergent crystals.
2010-08-25
yybbll
发件人： rui
发送时间： 2010-08-25 20:37:18
收件人： CCP4BB
抄送：
主题： [ccp4bb] turn granular to crystal
Hi, All,
I'm trying to crystallize a soluble protein and got something like granular,
they are rounded
