[ccp4bb] 10 mM Na Acetate buffer preparation

2012-02-03 Thread megha goyal
Our recombinant product is formulated in 10 mM sodium acetate buffer at pH 4.0 and the std composition mentions sodium 0.035 mg and acetate 0.59 mg per ml of sample. It mentions Sodium acetate is formed by titrating glacial acetic acid with sodium hydroxide. Can anyone guide me on how this 10 mM

Re: [ccp4bb] 10 mM Na Acetate buffer preparation

2012-02-03 Thread William G. Scott
http://en.wikipedia.org/wiki/Henderson–Hasselbalch_equation or any intro chemistry text William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Santa Cruz Santa Cruz, California

Re: [ccp4bb] 10 mM Na Acetate buffer preparation

2012-02-03 Thread Edward A. Berry
megha goyal wrote: Our recombinant product is formulated in 10 mM sodium acetate buffer at pH 4.0 and the std composition mentions sodium 0.035 mg and acetate 0.59 mg per ml of sample. It mentions Sodium acetate is formed by titrating glacial acetic acid with sodium hydroxide. Can anyone