[ccp4bb] A challenging Molecular replacement

2017-07-18 Thread CDaddy
I am very happy that our colleagues give a lot of valuable suggestions. Sometimes you need to select a good example to teach students. In this regard 5XQL seems a really unbelievable paradigm--poor data, rookie crystallographer, challenging molecular replacement and overnight accepted

Re: [ccp4bb] A challenging Molecular replacement

2017-07-17 Thread chenzhonghao...@163.com
. chenzhonghao...@163.com From: Tanner, John J. Date: 2017-07-18 00:34 To: CCP4BB Subject: Re: [ccp4bb] A challenging Molecular replacement Richard, I can’t help you with 5XQL. However, I can point out a recent structure from my group that might be useful for teaching. The structure was solved by MR

Re: [ccp4bb] A challenging Molecular replacement

2017-07-17 Thread Tanner, John J.
Richard, I can’t help you with 5XQL. However, I can point out a recent structure from my group that might be useful for teaching. The structure was solved by MR with a search model that had 33% sequence identity and represented only 46% of the target structure. The Methods section of the

Re: [ccp4bb] A challenging Molecular replacement

2017-07-17 Thread Ethan Merritt
On Tuesday, 18 July 2017 00:01:59 CDaddy wrote: > I am a structural biologist who is teaching X-ray crystallography. Recently I > noticed that BrlR structure (5XQL) was solved using molecular replacement > with a search model of very low similarity. I am very interested in this > structure

[ccp4bb] A challenging Molecular replacement

2017-07-17 Thread CDaddy
I am a structural biologist who is teaching X-ray crystallography. Recently I noticed that BrlR structure (5XQL) was solved using molecular replacement with a search model of very low similarity. I am very interested in this structure because I think this a very good example to show students